The theme song is the musical opening to every episode in the show, Teenage Mutant Ninja Turtles. The new main title sequence was showcased for the first time at the San Diego Comic-Con in 2012. The song is a remake of the original Turtle cartoon opening theme.
- The first few seconds of the opening is clips and lyrics are essentially identical to the 1987 Ninja Turtles cartoon.
- The theme tune toll call lists the Turtles in the exact same order as those of the 1987 cartoon and the Fast Forward and Back to the Sewer seasons of the 2003 cartoon: Leonardo, Donatello, Raphael, and Michelangelo.
- In addition, when the Turtles first start doing weapon demonstrations is a visual gag to 2003's second opening version.
- The phrase "Secret of the Ooze" is utilized within the theme. That phrase is the title of the second live-action Teenage Mutant Ninja Turtles movie that debuted in the late 1990's
- At the end of the theme, the Turtles all stand in a pose that mimics the first issue of Eastman and Laird's Teenage Mutant Ninja Turtles comic.